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Forty-eight freshly excised human heart specimens were divided into five groups as follows: control group, local STEMI group, local peri-infarction ischemia group, adjacent peri-infarction ischemia group and remote peri-infarction ischemia group. The morphology of coronary microvasculatures was observed by scanning electron microscopy. The expression of hypoxia-induced angiogenesis marker, vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1α (HIF-1α) and the ischemia-induced angiogenesis marker intercellular cell adhesion molecule-1 (ICAM-1) and CD-31 were detected by immunohistochemistry. Meanwhile, the expression of heart/brain natriuretic peptide (H/BNP) and tumor necrosis factor-α (TNF-α) were determined by western blot. VEGF and ICAM-1 positive expression increased in hypoxia induced angiogenesis. 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